The DNA metabarcoding spring school is now in its fourth edition. DNA metabarcoding is a rapidly evolving technique for assessing biodiversity from environmental DNA. Its range of applications is wide and covers, as examples, biodiversity monitoring, animal diet assessment and paleo-ecology. DNA metabarcoding relies on up-to-date molecular techniques such as PCR and next generation sequencing, and requires bioinformatic and biostatistic competencies to be able to analyse the results. This is intrinsically a technique at the interface of several disciplines that requires a broad range of skills in addition to the classical ecology knowledge related to the specific research topic. Each participant will have to present a 12 minute talk on their work and the relevance of DNA metabarcoding to their work to other participants in order to stimulate discussions with other participants and with the presenters.